Everything about Peptide Sequence totally explained
Peptide sequence or
amino acid sequence is the order in which
amino acid residues, connected by
peptide bonds, lie in the chain in
peptides and
proteins. The sequence is generally reported from the N-terminal end containing free
amino group to the C-terminal end containing free
carboxyl group. Peptide sequence is often called
protein sequence if it represents the
primary structure of a
protein.
Sequence notation and applications
Many peptide sequences have been determined and recorded in
sequence databases. These databases may use various notations to describe the peptide sequence. The full names of the amino acids are rarely given; instead,
3-letter or 1-letter abbreviations are usually recorded for conciseness.
Several deductions can be made from the sequence itself. Long stretches of
hydrophobic residues may indicate
transmembrane helices. These helices may indicate the peptide is a
cell receptor. Certain residues indicate a
beta sheet area. If full-length protein sequence is available, it's possible to estimate the
isoelectric point of the protein. Methods for determining the peptide sequence include deduction from
DNA sequence,
Edman degradation, and
mass spectrometry.
Techniques in
sequence analysis can be applied to learn more about the peptide. These techniques generally consist of comparing the sequence to other sequences from sequence databases. Other sequences may have already been studied and determined to be significant. Findings about these sequences may be applicable to the sequence under investigation.
Further Information
Get more info on 'Peptide Sequence'.
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